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1 8c368a17 Daofeng Li
GSM500898_4hr_K4_rep1_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-H3K4me3; developmental stage=4hpf (dome/30%); fraction=ChIP (control); antibody=anti-H3
2 8c368a17 Daofeng Li
GSM500899_4hr_K27_rep1_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-H3K27me3; developmental stage=4hpf (dome/30%); fraction=ChIP (control); antibody=anti-H3
3 8c368a17 Daofeng Li
GSM500900_4hr_K36_rep1_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-H3K36me3; developmental stage=4hpf (dome/30%); fraction=ChIP (control); antibody=anti-H3
4 8c368a17 Daofeng Li
GSM500901_4hr_polII_rep1_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-PolII; developmental stage=4hpf (dome/30%); fraction=Input; antibody=none
5 8c368a17 Daofeng Li
GSM500902_4hr_K4_rep2_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-H3K4me3; developmental stage=4hpf (dome/30%); fraction=ChIP (control); antibody=anti-H3
6 8c368a17 Daofeng Li
GSM500903_4hr_K36_rep2_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-H3K27me3; developmental stage=4hpf (dome/30%); fraction=ChIP (control); antibody=anti-H3
7 8c368a17 Daofeng Li
GSM500904_4hr_K27_rep2_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-H3K36me3; developmental stage=4hpf (dome/30%); fraction=ChIP (control); antibody=anti-H3
8 8c368a17 Daofeng Li
GSM500905_4hr_polII_rep2_MA2Cscore	developmental stage=4hpf (dome/30%); fraction=ChIP (test); antibody=anti-PolII; developmental stage=4hpf (dome/30%); fraction=Input; antibody=none
9 8c368a17 Daofeng Li
GSM500906_256_K4_MA2Cscore	developmental stage=256 cell; fraction=ChIP (test); antibody=anti-H3K4me3; developmental stage=256 cell; fraction=ChIP (control); antibody=anti-H3
10 8c368a17 Daofeng Li
GSM500907_256_K27_MA2Cscore	developmental stage=256 cell; fraction=ChIP (test); antibody=anti-H3K27me3; developmental stage=256 cell; fraction=ChIP (control); antibody=anti-H3
11 8c368a17 Daofeng Li
GSM500908_256_K36_MA2Cscore	developmental stage=256 cell; fraction=ChIP (test); antibody=anti-H3K36me3; developmental stage=256 cell; fraction=ChIP (control); antibody=anti-H3
12 8c368a17 Daofeng Li
GSM500909_256_polII_MA2Cscore	developmental stage=256 cell; fraction=ChIP (test); antibody=anti-PolII; developmental stage=256 cell; fraction=Input; antibody=none
13 8c368a17 Daofeng Li
GSE26609_H3K4me3_sp_ChIP	GSE=GSE26609; PMID=21383318
14 8c368a17 Daofeng Li
GSM564427_neg_RZN001_score22_zv9_density	background=AB, wild type; developmental stage=unfertilized eggs
15 8c368a17 Daofeng Li
GSM564428_neg_RZN002_score22_zv9_density	background=AB, wild type; developmental stage=just fertilized one cell embryos
16 8c368a17 Daofeng Li
GSM564429_neg_RZN003_score22_zv9_density	background=AB, wild type; developmental stage=mixure of 16/32 cell embryos
17 8c368a17 Daofeng Li
GSM564430_neg_RZN004_score22_zv9_density	background=AB, wild type; developmental stage=mixure of 128/256 cell embryos
18 8c368a17 Daofeng Li
GSM564431_neg_RZN005_score22_zv9_density	background=AB, wild type; developmental stage=MBT stage embryo (3.5hpf)
19 8c368a17 Daofeng Li
GSM564432_neg_RZN006_score22_zv9_density	background=AB, wild type; developmental stage=post-MBT stage embryo (5.3hpf)
20 8c368a17 Daofeng Li
RNA_2-4cell	tissue=embryo; developmental stage=embryogenesis: 2-4 cell stage; average insert size (fragment length)=710.502832; stdev for insert size=1336.039246
21 8c368a17 Daofeng Li
RNA_1Kcell	tissue=embryo; developmental stage=embryogenesis: 1K cell stage; average insert size (fragment length)=725.22487; stdev for insert size=1421.948
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RNA_dome	tissue=embryo; developmental stage=embryogenesis: dome stage; average insert size (fragment length)=663.190661; stdev for insert size=1281.290866
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RNA_shield	tissue=embryo; developmental stage=embryogenesis: shield stage; average insert size (fragment length)=297.987328; stdev for insert size=154.825595
24 8c368a17 Daofeng Li
RNA_bud	tissue=embryo; developmental stage=embryogenesis: bud stage; average insert size (fragment length)=606.819125; stdev for insert size=1119.538881
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RNA_28hpf	tissue=embryo; developmental stage=embryogenesis: 28 hpf stage; average insert size (fragment length)=627.757794; stdev for insert size=1180.386961
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RNA_2dpf	tissue=embryo; developmental stage=embryogenesis: 2 dpf stage; average insert size (fragment length)=610.692695; stdev for insert size=1125.321887
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RNA_5dpf	tissue=embryo; developmental stage=embryogenesis: 5 dpf stage; average insert size (fragment length)=1287.552595; stdev for insert size=1824.757762
28 8c368a17 Daofeng Li
H3K4me3_shield	developmental stage=embryo; embryogenesis stage=shield; chip antibody=Anti-trimethyl-Histone H3 (Lys4); chip antibody vendor=Millipore; chip antibody catalog number=#07-473
29 8c368a17 Daofeng Li
H3K27me3_shield	developmental stage=embryo; embryogenesis stage=shield; chip antibody=Anti-trimethyl-Histone H3 (Lys27); chip antibody vendor=Millipore; chip antibody catalog number=#07-449
30 8c368a17 Daofeng Li
ChIP_WCE_shield	developmental stage=embryo; embryogenesis stage=shield
31 8c368a17 Daofeng Li
GSM675174_MBT_H3K27me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K27me3; vendor=Millipore 07-449; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
32 8c368a17 Daofeng Li
GSM675175_MBT_H3K27me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K27me3; vendor=Millipore 07-449; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
33 8c368a17 Daofeng Li
GSM675176_MBT_H3K36me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K36me3; vendor=Diagenode pAb-058-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
34 8c368a17 Daofeng Li
GSM675177_MBT_H3K36me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K36me3; vendor=Diagenode pAb-058-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
35 8c368a17 Daofeng Li
GSM675178_MBT_H3K4me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K4me3; vendor=Diagenode pAb-003-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
36 8c368a17 Daofeng Li
GSM675179_MBT_H3K4me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K4me3; vendor=Diagenode pAb-003-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
37 8c368a17 Daofeng Li
GSM675180_MBT_H3K9me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K9me3; vendor=Diagenode pAb-056-50; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
38 8c368a17 Daofeng Li
GSM675181_MBT_H3K9me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=H3K9me3; vendor=Diagenode pAb-056-50; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
39 8c368a17 Daofeng Li
GSM675182_MBT_polii_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=RNA Pol II; vendor=Santa Cruz sc-899; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
40 8c368a17 Daofeng Li
GSM675183_MBT_polii_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; chip antibody=RNA Pol II; vendor=Santa Cruz sc-899; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none
41 8c368a17 Daofeng Li
GSM675184_postMBT_H3K27me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K27me3; vendor=Millipore 07-449; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
42 8c368a17 Daofeng Li
GSM675185_postMBT_H3K27me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K27me3; vendor=Millipore 07-449; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
43 8c368a17 Daofeng Li
GSM675186_postMBT_H3K36me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K36me3; vendor=Diagenode pAb-058-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
44 8c368a17 Daofeng Li
GSM675187_postMBT_H3K36me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K36me3; vendor=Diagenode pAb-058-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
45 8c368a17 Daofeng Li
GSM675188_postMBT_H3K4me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K4me3; vendor=Diagenode pAb-003-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
46 8c368a17 Daofeng Li
GSM675189_postMBT_H3K4me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K4me3; vendor=Diagenode pAb-003-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
47 8c368a17 Daofeng Li
GSM675190_postMBT_H3K9me3_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K9me3; vendor=Diagenode pAb-056-50; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
48 8c368a17 Daofeng Li
GSM675191_postMBT_H3K9me3_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=H3K9me3; vendor=Diagenode pAb-056-50; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
49 8c368a17 Daofeng Li
GSM675192_postMBT_polii_1	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=RNA Pol II; vendor=Santa Cruz sc-899; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
50 8c368a17 Daofeng Li
GSM675193_postMBT_polii_2	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; chip antibody=RNA Pol II; vendor=Santa Cruz sc-899; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none
51 8c368a17 Daofeng Li
GSM675194_preMBT_H3K27me3	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; chip antibody=H3K27me3; vendor=Millipore 07-449; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none
52 8c368a17 Daofeng Li
GSM675195_preMBT_H3K36me3	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; chip antibody=H3K36me3; vendor=Diagenode pAb-058-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none
53 8c368a17 Daofeng Li
GSM675196_preMBT_H3K4me3	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; chip antibody=H3K4me3; vendor=Diagenode pAb-003-050; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none
54 8c368a17 Daofeng Li
GSM675197_preMBT_H3K9me3	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; chip antibody=H3K9me3; vendor=Diagenode pAb-056-50; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none
55 8c368a17 Daofeng Li
GSM675198_preMBT_polii	cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; chip antibody=RNA Pol II; vendor=Santa Cruz sc-899; cell type=Embryonic cells; source=AB strain zebrafish embryos; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none
56 8c368a17 Daofeng Li
GSM813749_H3K4me3_IP_24hpf	tissue=whole embryo; developmental stage=24 hours post fertilization; genotype=wt; antibody=anti-H3K4me3; antibody manufacturer=Abcam; antibody catalog #=ab8580
57 8c368a17 Daofeng Li
GSM813750_H3K4me3_IP_72hpf	tissue=whole embryo; developmental stage=72 hours post fertilization; genotype=wt; antibody=anti-H3K4me3; antibody manufacturer=Abcam; antibody catalog #=ab8580
58 8c368a17 Daofeng Li
GSM813751_H3K4me3_IP_Adult	tissue=whole adult; developmental stage=mixed gender adults; genotype=wt; antibody=anti-H3K4me3; antibody manufacturer=Abcam; antibody catalog #=ab8580
59 8c368a17 Daofeng Li
GSM813752_H3K36me3_IP_24hpf	tissue=whole embryo; developmental stage=24 hours post fertilization; genotype=wt; antibody=anti-H3K36me3; antibody manufacturer=Abcam; antibody catalog #=ab9050
60 8c368a17 Daofeng Li
GSM813753_H3K36me3_IP_72hpf	tissue=whole embryo; developmental stage=72 hours post fertilization; genotype=wt; antibody=anti-H3K36me3; antibody manufacturer=Abcam; antibody catalog #=ab9050
61 8c368a17 Daofeng Li
GSM813754_H3K36me3_IP_Adult	tissue=whole adult; developmental stage=mixed gender adults; genotype=wt; antibody=anti-H3K36me3; antibody manufacturer=Abcam; antibody catalog #=ab9050
62 8c368a17 Daofeng Li
GSM853234_Nanog_3.5hpf	tissue=embryos; developmental stage=3.5hpf; strain=TU; chip antibody=Myc; binding site=Nanog-like
63 8c368a17 Daofeng Li
GSM853235_Nanog_4.5hpf	tissue=embryos; developmental stage=4.5hpf; strain=TU; chip antibody=Myc; binding site=Nanog-like
64 8c368a17 Daofeng Li
GSM853236_Mxtx_4.5hpf	tissue=embryos; developmental stage=4.5hpf; strain=TU; chip antibody=Myc; binding site=Mxtx2
65 8c368a17 Daofeng Li
H3K4me3_dome.norm	tissue=whole embryo; developmental stage=dome; antibody=H3K4me3; antibody vendor=Diagenode; antibody catalog=pAb-003-050; antibody batch=A3152-001P
66 8c368a17 Daofeng Li
H3K4me3_80epi.norm	tissue=whole embryo; developmental stage=80% epiboly; antibody=H3K4me3; antibody vendor=Diagenode; antibody catalog=pAb-003-050; antibody batch=A3152-001P
67 8c368a17 Daofeng Li
H3K4me3_24hpf.norm	tissue=whole embryo; developmental stage=24hpf; antibody=H3K4me3; antibody vendor=Diagenode; antibody catalog=pAb-003-050; antibody batch=A3152-001P
68 8c368a17 Daofeng Li
H3K4me3_48hpf.norm	tissue=whole embryo; developmental stage=48hpf; antibody=H3K4me3; antibody vendor=Diagenode; antibody catalog=pAb-003-050; antibody batch=A3152-001P
69 8c368a17 Daofeng Li
H3K4me1_dome.norm	tissue=whole embryo; developmental stage=dome; antibody=H3K4me1; antibody vendor=Diagenode; antibody catalog=CS-037-100; antibody batch=A399-001
70 8c368a17 Daofeng Li
H3K4me1_80epi.norm	tissue=whole embryo; developmental stage=80% epiboly; antibody=H3K4me1; antibody vendor=Diagenode; antibody catalog=CS-037-100; antibody batch=A399-001
71 8c368a17 Daofeng Li
H3K4me1_24hpf.norm	tissue=whole embryo; developmental stage=24hpf; antibody=H3K4me1; antibody vendor=Diagenode; antibody catalog=CS-037-100; antibody batch=A399-001
72 8c368a17 Daofeng Li
H3K4me1_48hpf.norm	tissue=whole embryo; developmental stage=48hpf; antibody=H3K4me1; antibody vendor=Diagenode; antibody catalog=CS-037-100; antibody batch=A399-001
73 8c368a17 Daofeng Li
H3K27ac_dome.norm	tissue=whole embryo; developmental stage=dome; antibody=H3K27ac; antibody=Abcam; antibody catalog=ab4729; antibody batch=GR6802
74 8c368a17 Daofeng Li
H3K27ac_80epi.norm	tissue=whole embryo; developmental stage=80% epiboly; antibody=H3K27ac; antibody=Abcam; antibody catalog=ab4729; antibody batch=GR6802
75 8c368a17 Daofeng Li
H3K27ac_24hpf.norm	tissue=whole embryo; developmental stage=24hpf; antibody=H3K27ac; antibody=Abcam; antibody catalog=ab4729; antibody batch=GR6802
76 8c368a17 Daofeng Li
H3K27ac_48hpf.norm	tissue=whole embryo; developmental stage=48hpf; antibody=H3K27ac; antibody=Abcam; antibody catalog=ab4729; antibody batch=GR6802
77 8c368a17 Daofeng Li
GSM822655_preMBT_MeDIP_1	cell type=Embryonic cells; strain=AB; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
78 8c368a17 Daofeng Li
GSM822656_preMBT_MeDIP_2	cell type=Embryonic cells; strain=AB; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=preMBT, 2.5 hpf, 256-cell stage; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
79 8c368a17 Daofeng Li
GSM822657_MBT_MeDIP_1	cell type=Embryonic cells; strain=AB; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
80 8c368a17 Daofeng Li
GSM822658_MBT_MeDIP_2	cell type=Embryonic cells; strain=AB; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=MBT, 3.5 hpf, 1000+-cell stage; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
81 8c368a17 Daofeng Li
GSM822659_postMBT_MeDIP_1	cell type=Embryonic cells; strain=AB; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
82 8c368a17 Daofeng Li
GSM822660_postMBT_MeDIP_2	cell type=Embryonic cells; strain=AB; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=postMBT, 5.3 hpf, 50% epiboly; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
83 8c368a17 Daofeng Li
GSM822661_ZF4_MeDIP_1	cell line=ZF4; biomaterial provider=American Type Culture Collection; developmental stage=Embryo; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Cells were harvesdted by trypsination and flash frozen in liq. N2 as dry pellets; cell line=ZF4; biomaterial provider=American Type Culture Collection; developmental stage=Embryo; antibody=none, input; sample preparation protocol=Cells were harvesdted by trypsination and flash frozen in liq. N2 as dry pellets
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GSM822662_ZF4_MeDIP_2	cell line=ZF4; biomaterial provider=American Type Culture Collection; developmental stage=Embryo; antibody=Anti-5-methylcytosine antibody (10 ng/µl); antibody catalog#=Mab-006-100; antibody vendor=Diagenode; sample preparation protocol=Cells were harvesdted by trypsination and flash frozen in liq. N2 as dry pellets; cell line=ZF4; biomaterial provider=American Type Culture Collection; developmental stage=Embryo; antibody=none, input; sample preparation protocol=Cells were harvesdted by trypsination and flash frozen in liq. N2 as dry pellets
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GSM913999_sperm_MeDIP_1	cell type=Sperm; strain=AB; developmental stage=Sperm [from adult males]; medip antibody=Anti-5-methylcytosine antibody (10 ng/µl); medip antibody catalog#=Mab-006-100; medip antibody vendor=Diagenode; sample preparation protocol=Zebrafish males were starved for 24 hrs and anesthetized in fish water with 0.016% tricaine (Sigma, Cat. No A-5040) immediately before sperm collection. Sperm was obtained with a capillary tube under gentle suction and flash frozen in liq. N2. Prior to DNA isolation, sperm was washed three times in T10N150E10 buffer (10 mM Tris-HCl pH 7.8, 150 mM NaCl,10 mM EDTA pH 8.0); cell type=Sperm; strain=AB; developmental stage=Sperm [from adult males]; antibody=none, input; sample preparation protocol=Zebrafish males were starved for 24 hrs and anesthetized in fish water with 0.016% tricaine (Sigma, Cat. No A-5040) immediately before sperm collection. Sperm was obtained with a capillary tube under gentle suction and flash frozen in liq. N2. Prior to DNA isolation, sperm was washed three times in T10N150E10 buffer (10 mM Tris-HCl pH 7.8, 150 mM NaCl,10 mM EDTA pH 8.0)
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GSM914000_sperm_MeDIP_2	cell type=Sperm; strain=AB; developmental stage=Sperm [from adult males]; medip antibody=Anti-5-methylcytosine antibody (10 ng/µl); medip antibody catalog#=Mab-006-100; medip antibody vendor=Diagenode; sample preparation protocol=Zebrafish males were starved for 24 hrs and anesthetized in fish water with 0.016% tricaine (Sigma, Cat. No A-5040) immediately before sperm collection. Sperm was obtained with a capillary tube under gentle suction and flash frozen in liq. N2. Prior to DNA isolation, sperm was washed three times in T10N150E10 buffer (10 mM Tris-HCl pH 7.8, 150 mM NaCl,10 mM EDTA pH 8.0); cell type=Sperm; strain=AB; developmental stage=Sperm [from adult males]; antibody=none, input; sample preparation protocol=Zebrafish males were starved for 24 hrs and anesthetized in fish water with 0.016% tricaine (Sigma, Cat. No A-5040) immediately before sperm collection. Sperm was obtained with a capillary tube under gentle suction and flash frozen in liq. N2. Prior to DNA isolation, sperm was washed three times in T10N150E10 buffer (10 mM Tris-HCl pH 7.8, 150 mM NaCl,10 mM EDTA pH 8.0)
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GSM914001_emb24h_MeDIP_1	cell type=Embryonic cells; strain=AB; developmental stage=24 hpf embryo, pharyngula stage; medip antibody=Anti-5-methylcytosine antibody (10 ng/µl); medip antibody catalog#=Mab-006-100; medip antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=24 hpf embryo, pharyngula stage; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
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GSM914002_emb24h_MeDIP_2	cell type=Embryonic cells; strain=AB; developmental stage=24 hpf embryo, pharyngula stage; medip antibody=Anti-5-methylcytosine antibody (10 ng/µl); medip antibody catalog#=Mab-006-100; medip antibody vendor=Diagenode; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.; cell type=Embryonic cells; strain=AB; developmental stage=24 hpf embryo, pharyngula stage; antibody=none, input; sample preparation protocol=Chorions were removed with 1 mg/ml pronase and embryos washed in egg water. Yolk proteins were removed in a de-yolking buffer, embryos were sedimented and flash-frozen.
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BisSeq01_sperm.bedGraph	SRX=SRX257162
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BisSeq02_egg.bedGraph	SRX=SRX257163
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BisSeq03_2-16cell.bedGraph	SRX=SRX257164
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BisSeq04_64cell.bedGraph	SRX=SRX257166
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BisSeq05_256cell.bedGraph	SRX=SRX257168
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BisSeq06_sphere.bedGraph	SRX=SRX257169
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BisSeq07_muscle.bedGraph	SRX=SRX257172
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BisSeq09_sphere_parthenogenesis_control.bedGraph	SRX=SRX257188
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BisSeq08_sphere_parthenogenesis_treatment.bedGraph	SRX=SRX257193
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GSM1133391_chrall.sperm.bedGraph	cell type=sperm; developmental stage=gamete; stain=Tubingen; library strategy=BS-Seq
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GSM1133392_chrall.egg.bedGraph	cell type=egg; developmental stage=gamete; stain=Tubingen; library strategy=BS-Seq
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GSM1133393_chrall.16-cell.bedGraph	cell type=16-cell; developmental stage=embryo; stain=Tubingen; library strategy=BS-Seq
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GSM1133394_chrall.32-cell.bedGraph	cell type=32-cell; developmental stage=embryo; stain=Tubingen; library strategy=BS-Seq
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GSM1133395_chrall.64-cell.bedGraph	cell type=64-cell; developmental stage=embryo; stain=Tubingen; library strategy=BS-Seq
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GSM1133396_chrall.128-cell.bedGraph	cell type=128-cell; developmental stage=embryo; stain=Tubingen; library strategy=BS-Seq
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GSM1133397_chrall.1k-cell.bedGraph	cell type=1k-cell; developmental stage=embryo; stain=Tubingen; library strategy=BS-Seq
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GSM1133398_chrall.Germring.bedGraph	cell type=Germring; developmental stage=embryo; stain=Tubingen; library strategy=BS-Seq
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GSM1133399_chrall.testis.bedGraph	cell type=testis; developmental stage=organ; stain=Tubingen; library strategy=BS-Seq